contextual fear conditioning cfc test  (Med Associates Inc)

Journal: Experimental Animals

Article Title: Middle aged CAMKII-Cre: Cbs fl/fl mice: a new model for studying perioperative neurocognitive disorders

doi: 10.1538/expanim.23-0065

Figure Lengend Snippet: Anesthesia and surgery induced significant cognitive deficits in 8-month-old CAMKII-Cre: Cbs fl/fl mice. (A) Experimental timeline of surgery and behavioral tests. (B) Representative movement tracks in the open-field test. (C) Time spent in the central zone in the open-field test (ns: no significant difference, mean ± SD, One-way ANOVA). WT: n=8, Littermate: n=6, CBS-CKO: n=7. (D) Locomotive ability was evaluated by the average speed in the open-field test (ns: no significant difference, mean ± SD, one-way ANOVA). (E) and (F) show the statistical results of spatial learning (mean ± SEM, two-way ANOVA, ** P< 0.01, **** P< 0.0001) during four consecutive training days and spatial memory (mean ± SD, one-way ANOVA, **** P< 0.0001) on the test day of the Barnes maze test. (G) Contextual memory was also evaluated by the ratio of freezing time in the contextual fear conditioning test (mean ± SD, one-way ANOVA, *** P< 0.001, **** P< 0.0001).

Article Snippet: Contextual fear conditioning test: Contextual fear memory was analyzed by using a Ugo Basile Fear Conditioning System (Ugo Basile SRL, Gemonio, Italy).

Techniques:

Journal: Experimental Animals

Article Title: Middle aged CAMKII-Cre: Cbs fl/fl mice: a new model for studying perioperative neurocognitive disorders

doi: 10.1538/expanim.23-0065

Figure Lengend Snippet: 8-month-old CAMKII-Cre: Cbs fl/fl mice demonstrated similar cognitive deficits to 18-month-old wild type counterparts after undergoing exploratory laparotomy. (A) Experimental timeline of surgery and behavioral tests. (B) Representative movement tracks in the open-field test. (C) Time spent in the central zone in the open-field test (ns: no significant difference, mean ± SD, unpaired t -test). PND: n=8, CBS-CKO: n=7. (D) Locomotive ability was evaluated by the average speed in the open field test (ns: no significant difference, mean ± SD, unpaired t -test). (E) and (F) show the statistical results of spatial learning (ns: no significant difference, mean ± SEM, two-way ANOVA) during four consecutive days and spatial memory (ns: no significant difference, mean ± SD, unpaired t -test) on the test day of the Barnes maze tests. (G) Contextual memory was also evaluated by the ratio of freezing time in the contextual fear conditioning test (ns: no significant difference, mean ± SD, unpaired t -test).

Article Snippet: Contextual fear conditioning test: Contextual fear memory was analyzed by using a Ugo Basile Fear Conditioning System (Ugo Basile SRL, Gemonio, Italy).

Techniques:

Journal: Science (New York, N.Y.)

Article Title: Female-specific synaptic dysfunction and cognitive impairment in a mouse model of PCDH19 disorder

doi: 10.1126/science.aaz3893

Figure Lengend Snippet: (A to G) Fear conditioning test. (A) Schematic of the contextual and cued fear conditioning test. Context A is different from context B. CS, white noise; US, 0.3 mA foot shock. (B to G) Freezing responses of female (B to D) and male (E to G) mice. (B and E) Freezing responses on day 1. CS was presented for 30 s, 3 times, (gray shadow) and at the end of each CS, a US was given (red). (C and F) Freezing responses on day 2 (contextual test). (D and G) Freezing responses on day 3 (cued test). CS was presented at 3–6 min (gray shadow). Freezing responses during the conditioning, contextual and cued tests were similar between Pcdh19 mutant and control mice. n = 14 mice for Pcdh19WT♀, 10 for Pcdh19HET♀, 12 for Pcdh19WT♂, and 12 for Pcdh19HEMI♂.

Article Snippet: Contextual and cued fear conditioning tests, pre-exposure dependent fear facilitation (Pattern completion) task, contextual discrimination fear learning (Pattern separation) task, and distinct contextual fear conditioning tests were conducted in a conditioning chamber (Ugo Basile) equipped with a digital video camera and a sound-attenuating chest.

Techniques: Mutagenesis, Control

Journal: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology

Article Title: Psychiatric-disorder-related behavioral phenotypes and cortical hyperactivity in a mouse model of 3q29 deletion syndrome.

doi: 10.1038/s41386-019-0441-5

Figure Lengend Snippet: Fig. 2 Psychiatric disorder-related behavioral phenotypes in Df/+ mice. a Increased startle amplitude in Df/+ mice (WT, n = 10; Df/+, n = 10). *P < 0.05, one-way analysis of variance (ANOVA). WT, wild-type littermates. b Impaired prepulse inhibition (PPI) in Df/+ mice (WT, n = 10; Df/+, n = 10). *P < 0.05, repeated two-way ANOVA with Bonferroni post hoc tests. c Impaired social interaction in Df/+ mice (WT, n = 10; Df/+, n = 10). *P < 0.05, one-way ANOVA. d Increased self-grooming activity (an index of repetitive behavior) in Df/+ mice (WT, n = 10; Df/+, n = 10). *P < 0.05, one-way ANOVA. e Impaired fear conditioning in Df/+ mice (WT, n = 10; Df/+, n = 9). *P < 0.05, one-way ANOVA. f Increased open- field activity in Df/+ mice (WT, n = 8; Df/+, n = 9). *P < 0.05, one-way ANOVA. g Time in the center zone during the first 5 min in the open-field test (WT, n = 8; Df/+, n = 9). h Rescue of the increased startle amplitude by risperidone administration in Df/+ mice (each n = 10). *P < 0.05, **P < 0.01, two-way ANOVA with Bonferroni post hoc tests. i Rescue of the impaired PPI by risperidone administration in Df/+ mice (each n = 10). *P < 0.05, two-way ANOVA with Bonferroni post hoc tests. The percent of the PPI at all dB levels was not significantly different between risperidone-treated Df/+ and WT mice (68 dB, P = 0.557; 71 dB, P = 0.326; 74 dB, P = 0.318). Data are presented as the mean ± s.e.m.

Article Snippet: Contextual fear conditioning test Fear conditioning was conducted in a fear conditioning chamber (30 × 24 × 21 cm3, Med Associates Inc., VT, USA).

Techniques: Inhibition, Activity Assay